Development of a sensitive HPLC method with fluorescence detection for determination of motilin in human plasma

Karaca S. A., TEMEL T., YENİCELİ UĞUR D.

BIOANALYSIS, cilt.15, ss.219-229, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15
  • Basım Tarihi: 2023
  • Doi Numarası: 10.4155/bio-2023-0003
  • Dergi Adı: BIOANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.219-229
  • Anahtar Kelimeler: chromatography, experimental design, motilin, peptide, quantification, LIQUID-CHROMATOGRAPHY, PEPTIDES, GHRELIN
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Background: Motilin is a peptide-structured gastrointestinal system hormone. In this study, a sensitive HPLC-fluorescence detection method was developed and validated for the quantification of motilin in human plasma. Materials & methods: Optimization processes were carried out with the experimental design methodology. Analyses were performed on a C8 column (4.6 x 150 mm, 3.5 mu m particles) using water and acetonitrile containing trifluoroacetic acid as the mobile phase. Results & conclusion: The method was linear from 2 to 200 ng/ml of motilin. The assay variability was less than 5%. The limit of quantification was found to be 1.84 ng/ml. The applicability of the developed method was successfully demonstrated by quantifying the levels of motilin in human plasma samples.