In Vitro Regeneration Potential of Lotus maritimus L. (Leguminosae)

GÖKÇE P., AVCI S.

KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, cilt.23, sa.4, ss.870-875, 2020 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 4
  • Basım Tarihi: 2020
  • Doi Numarası: 10.18016/ksutarimdoga.vi.657729
  • Dergi Adı: KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.870-875
  • Anahtar Kelimeler: Epicotyl, Hypocotyl, Callus induction, NAA concentrations, Kinetin, PLANT-REGENERATION, TRANSFORMATION, JAPONICUS, CULTURE, TENUIS, CALLUS
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

To investigate the regeneration potential of Lotus maritimus L., the stem, hypocotyl, cotyledon, root, and epicotyl were cultured in MS media containing different combinations of alpha-naphthalene acetic acid (NAA) concentrations (1, 2, and 4 mg L-1) and cytokinin types [0 (control), 1 mg L-1 of 6-Benzilaminopurin (I3AP), and 0.5 mg L.1 of kinetin]. The highest callus induction was achieved with the stem and epicotyl explants at 1 or 2 mg L-1 concentrations of NAA plus 0.5 mg L-1 of kinetin. The best plant regeneration values (3.56 and 3.20 shoots per callus) were del ermined with the epicotyl and hypocotyl cultured in MS media containing 1 mg LI of NAA. The obtained shoots were easily rooted in half-strength MS media containing 1 mg L-1 NAA and substantially adapted to the external environment.