In Vitro Regeneration Potential of Lotus maritimus L. (Leguminosae)


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Gokce P., Avci S.

KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, cilt.23, sa.4, ss.870-875, 2020 (ESCI, TRDizin) identifier

Özet

To investigate the regeneration potential of Lotus maritimus L., the stem, hypocotyl, cotyledon, root, and epicotyl were cultured in MS media containing different combinations of alpha-naphthalene acetic acid (NAA) concentrations (1, 2, and 4 mg L-1) and cytokinin types [0 (control), 1 mg L-1 of 6-Benzilaminopurin (I3AP), and 0.5 mg L.1 of kinetin]. The highest callus induction was achieved with the stem and epicotyl explants at 1 or 2 mg L-1 concentrations of NAA plus 0.5 mg L-1 of kinetin. The best plant regeneration values (3.56 and 3.20 shoots per callus) were del ermined with the epicotyl and hypocotyl cultured in MS media containing 1 mg LI of NAA. The obtained shoots were easily rooted in half-strength MS media containing 1 mg L-1 NAA and substantially adapted to the external environment.