The synergistic effects of notch-1, IL-1β and leptin on migration and angiogenesis in colorectal cancer
CUKUROVA MEDICAL JOURNAL, cilt.51, sa.2, ss.448-458, 2026 (ESCI, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 51 Sayı: 2
- Basım Tarihi: 2026
- Doi Numarası: 10.17826/cumj.1848821
- Dergi Adı: CUKUROVA MEDICAL JOURNAL
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Directory of Open Access Journals, TR DİZİN (ULAKBİM), Academic Search Ultimate (EBSCO)
- Sayfa Sayıları: ss.448-458
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Eskişehir Osmangazi Üniversitesi Adresli: Evet
Özet
Purpose: The aim of this study was to investigate the synergistic interaction of Notch-1, interleukin-1 beta, and leptin on each other, cell migration, and proangiogenic signaling in colorectal cancer cell lines. Materials and Methods: Human colorectal cancer cell lines, HCT-116 and HCT-15, were used. The genes for Notch-1, interleukin-1 beta, and leptin were silenced using small interfering ribonucleic acid. The efficacy of gene silencing and the messenger ribonucleic acid levels of target genes, including Notch-1, interleukin-1 beta, interleukin-1 receptor, leptin, vascular endothelial growth factor A, vascular endothelial growth factor receptor 2, and matrix metalloproteinase-2, were analyzed via quantitative real-time polymerase chain reaction. Protein levels of interleukin-1 beta, leptin, and vascular endothelial growth factor A were also measured using enzyme-linked immunosorbent assay. Results: In the Notch-1 inhibition group, significant decreases were observed in the messenger ribonucleic acid levels of Notch-1, interleukin-1 beta, interleukin-1 receptor (a 3.45-fold decrease), leptin, vascular endothelial growth factor receptor 2, and matrix metalloproteinase-2 (a 6.36-fold decrease). Leptin inhibition led to reduced messenger ribonucleic acid levels of Notch-1, interleukin-1 beta (a 3.67-fold decrease), interleukin-1 receptor (a 2.53-fold decrease), leptin, vascular endothelial growth factor A, and matrix metalloproteinase-2 (a 3.22-fold decrease). Conclusion: Notch-1, interleukin-1 beta, and leptin interact with each other, directly contributing to cell migration and the expression of angiogenic molecules in colorectal cancer.