Akademik Veri Yönetim Sistemi
International Conference on Chemical, Agricultural, Biological and Environmental Sciences , Berlin, Almanya, 3 - 04 Ağustos 2023, ss.12
Background/Objectives: Type 2 (non-insulin-dependent) diabetes is one of the most common metabolic disorders characterized by decreased insulin secretion caused by pancreatic β-cell dysfunction and insulin resistance in target organs. Since insulin release and activity are essential processes for glucose homeostasis, the molecular mechanisms involved in the synthesis and release of insulin are tightly regulated. Defects in the mechanisms involved in these processes can lead to a metabolic imbalance responsible for the development of the disease. Type 2 diabetes accounts for more than 90% of patients with diabetes and leads to microvascular and macrovascular complications that cause profound psychological and physical distress to patients and place a huge burden on healthcare systems. Despite growing knowledge of risk factors for type 2 diabetes and evidence of successful prevention programs, the incidence, and prevalence of the disease continue to increase globally. Early diagnosis and availability of safe and effective treatments through screening programs reduce morbidity and mortality by preventing or delaying complications. A better understanding of the specific diabetes phenotypes and genotypes may facilitate the early diagnosis of patients with type 2 diabetes.
Phosphatase and tensin homolog (PTEN) is a potent tumor suppressor gene that antagonizes the proto-oncogenic phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) signaling pathway and governs essential cellular metabolic processes. PTEN serves as a negative regulator of the PI3K/PTEN/Akt signaling pathway, which regulates cellular functions such as cell growth, differentiation, proliferation, and migration. PI3K/PTEN/Akt signaling cascades may also affect glucose uptake via translocation of GLUT-4. PTEN functions as a potent negative regulator of insulin signaling and is one of the possible candidates associated with susceptibility to the development of type 2 diabetes.
Methods: In light of this information, our study was planned to determine the relationship between the IVS4(-/+) polymorphism of the PTEN gene and the risk of Type-2 Diabetes. In our study; DNA was isolated from blood samples taken from 100 Type-2 Diabetes patients and 100 healthy individuals. The PCR-RFLP method was used for the evaluation of polymorphisms.
Results: 43(-/+), 57(-/-) genotypes were determined in diabetic patients for PTEN IVS4(-/+) variant, while 53(-/+), 47(-/-) genotypes were determined in control individuals (Table 1).
As a conclusion; When these data were evaluated, no statistically significant difference was found between the diabetic patients and the control group in the genotype distribution of the PTEN IVS4 (-/+) variant (p<0.05).