Comparison of Four Phenotypic Assays and Check-Direct CPE for Detection of Carbapenemases-Producing Enterobacterales


Abulaila A., Erdem F., ÖNCÜL M. O., AKTAŞ Z.

CLINICAL LABORATORY, vol.67, no.7, pp.1707-1715, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 67 Issue: 7
  • Publication Date: 2021
  • Doi Number: 10.7754/clin.lab.2020.201002
  • Journal Name: CLINICAL LABORATORY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE
  • Page Numbers: pp.1707-1715
  • Keywords: Enterobacterales, carbapenemase, OXA-48/NDM/KPC, phenotypic tests, real-time PCR, Check-Direct CPE, MODIFIED HODGE TEST, KLEBSIELLA-PNEUMONIAE, RAPID DETECTION, CARBA-NP, BLUE-CARBA, KPC, PCR
  • Eskisehir Osmangazi University Affiliated: No

Abstract

Background: Early and accurate detection of carbapenemase-producing Enterobacterales (CPE) is fundamental to prevent their spread in hospital environment. Our objective was to compare between four commonly used phenotypic assays and Check-Direct CPE (CDCPE) multiplex PCR in CPE detection. We examined stool samples or rectal swabs for CPE, samples collected from 23 Jan 2017 to 23 Jul 2017 from patients in intensive-care units (ICUs) of our hospital.