Akademik Veri Yönetim Sistemi
IV. International Scientific and Vocational Studies Congress – Engineering, Ankara, Türkiye, 7 - 10 Kasım 2019, ss.174-177
Recently, electrochemical methods are of growing interest. Electrochemical methods used in the development of sensors have many advantages such as high sensitivity, short response time, and low cost. Screen-printed electrodes (SPEs) are one of the electrochemical devices utilized as sensors that are used in a wide range of applications from health to food. In this study, the surface functionalization of commercially available screen-printed carbon electrodes (SPCE) were carried out, and the layers formed on SPCE surface were examined by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) method. Textural and micro-structural properties of SPCE were investigated by scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) spectroscopy. Bonding of a self-assembling monolayer (SAM), an antibody, and an antigen on SPCE were also successfully achieved. After each layer formation, EIS tests were performed using the redox probe potassium ferricyanide (K3Fe(CN)6) at the both optimum concentration and parameters. The impedance data were presented in the Nyquist curves. The Nyquist plot provides charge transfer resistance (Rct) when employed along with the equivalent circuit model. The results showed that the Rct values increased with the formation of additional layers. The increasing resistance showed a successful achievement of the immobilization process.