Aim: This study was conducted to determine midwives' professional belonging and burnout levels and the factors affecting these levels. Subject and methods: This multicenter cross-sectional explorative study included 2013 midwives reached between January 1 and June 30, 2018. A stratified sampling method was applied to reach a sample distribution that adequately represented Turkey. An introductory information form, Copenhagen Burnout Inventory, and Midwifery Belonging Scale were used as data collection tools. Data were analyzed by simple and multiple linear regression analysis. Results: It was found that the burnout levels of the midwives were affected by the work unit, work experience, and work schedule, while the levels of professional belonging among the midwives were affected by the burnout level, marital status, number of children, satisfaction of social circle with midwifery, reasons for choosing midwifery, work unit, and work experience. Conclusion: Managerial plans and interventions are needed to increase midwives' sense of professional belonging and improve their burnout level.