Screening of non-alkaloid acetylcholinesterase and carbonic anhydrase isoenzymes inhibitors of Leiotulus dasyanthus (K. Koch) Pimenov & Ostr. (Apiaceae)


KARAKAYA S., Bingol Z., KOCA M., DEMİRCİ B., GÜLÇİN İ., Baser K. H. C.

JOURNAL OF ESSENTIAL OIL RESEARCH, vol.32, no.3, pp.227-241, 2020 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 3
  • Publication Date: 2020
  • Doi Number: 10.1080/10412905.2020.1746415
  • Title of Journal : JOURNAL OF ESSENTIAL OIL RESEARCH
  • Page Numbers: pp.227-241

Abstract

The oxidant, cholinesterase, and carbonic anhydrase inhibitions of essential oils and their major compounds of Leiotulus dasyanthus and molecular docking studies of alpha-pinene and (TM)-3-carene were evaluated in this study. Phenolic constituents and antioxidant activity of samples were determined by Folin-Ciocalteu's, DPPH, and TBA processes. Cholinesterase inhibition effect was evaluated through Ellman's method. GC-FID and GC/MS were used for analysing of essential oils. The root oil got the highest total phenolic content (898.87 mg/g). The utmost activity was shown flower oil (29.31 mu g/mL) in the DPPH test. The root oil indicated considerable inhibition towards acetylcholinesterase (21.92%) and butyrylcholinesterase (91.33%) enzymes, respectively. alpha-pinene and delta-3-carene indicated high acetylcholinesterase inhibitory activity (66.44 and 48.71%, respectively). Cytosolic hCA I and II isoenzymes were influentially inhibited by flower and aerial part oils with 1.694 and 1.677 mu M IC50 values, respectively. 1-Dodecanol (7.8%); beta-pinene (63.3%), alpha-pinene (20.5%); octyl acetate (14.7%); hexyl octanoate (55.1%) were characterized as principle compounds of aerial parts, roots, flowers, and fruits respectively.