The aim of this paper is to evaluate the inactivation effect of indirect low temperature plasma exposure at atmospheric pressure produced by the original Low Temperature Atmospheric Pressure Plasma (LTAPP) system. In this system, the low temperature plasma is generated using a high voltage power supply in oxygen at atmospheric pressure. The obtained survivor curves for Escherichia coli (G(-)), Bacillus subtilis (G(+)), Staphylococcus aureus (G(+)), Candida albicans (Yeast), and Saccharomyces cerevisiae (Yeast) showed that the LTAPP system could inactivate them. The optical microscope images of B. subtilis and S. cerevisiae treated have confirmed the inactivation effect of the LTAPP system. (C) 2014 Elsevier B.V. All rights reserved.