Akademik Veri Yönetim Sistemi
GRASAS Y ACEITES, cilt.63, sa.3, ss.274-283, 2012 (SCI-Expanded)
An acidolysis reaction of triolein with caprylic and palmitic acids was performed using immobilized sn-1,3 specific lipase from Mucor miehei to produce a reduced calorie spreadable structured lipid (SL). Response surface methodology was applied to model and optimize the reaction conditions using a four-factor five-level central composite rotatable design. The selected factors were time (10-24 h), enzyme load (10-25 wt%), substrate mole ratio (Triolein:Caprylic acid:Palmitic acid), (1:1:1-1:2.5:2.5) and temperature (45-60 degrees C). The produced SLs were compared to fat extracts of commercial margarine in terms of melting profile and solid fat content (SFC). SL with a melting peak of 42 degrees C and SFC of 40.69% at 0 degrees C was very similar to soft margarines. The caloric value of this SL was determined as 37.74 kJ/g, theoretically. The optimum reaction conditions were found as reaction time 14 h; substrate mole ratio 1:2.1:2.1; temperature 58 degrees C; and enzyme load 15 wt%. Under optimum conditions, the product contained 29.68% COC, 25.47% POC, and 3.80% POP.