© 2022 Verlag Ferdinand Berger und Sohne GmbH. All rights reserved.This study aims to determine microfungal diversity (Moniliaceae family) in the Çamalti Saltern, Ibrkey. Water samples were seasonally taken from four stations in the Çamalti Saltern ponds. Microfungi were isolated by membrane filtration method using DRBC and DRBClO.The isolates have been identified using morphological and molecular techniques. The average numbers of all the fungal colonies were determined to be 14 CFU/100 ml and 28 CFU/100 ml on DRBC and DRBCIO media, respectively A total of 35 species belonging to 5 genera have been identified. These genera were Acremoniutn (1 species), Aspergillus (14 species), Fusarium (2 species). Pénicillium (16 species), and Tríchoderma (2 species). According to their relative abundance, the dominant genus isolated on DRBC and DRBCIO medium was Penicillium (47 %).This study notes that the aquatic mycobiota of Çamalti Saltern has a high diversity and most of the isolated species are inhabitants of hypersaline environments. Successful results were obtained by using DRBC and DRBCIO on the isolation of the species that have different salt requirements. Using molecular techniques as well as classical techniques, identification of microfungus has been complemental as the classical techniques are inadequate. This study represents the first survey of moniliaceous fungi in İzmir Çamalti Saltern and provides data on their diversity and distribution.