In this study, a factorial design was planned for removal of Remazol Black B dye by the fungus called A. niger isolated from Izmir camalti Saltpan in Turkey. Initial pH, molasses concentration, initial dye concentration, and the effect of salt on dye removal were examined at the batch scale level. The optimal dye concentration, pH, and molasses dosage were determined as 100 ppm, 3, and 10 g/L for the most effective decolorization rate, respectively. In this study, A. niger removed 98.97% of Remazol Black B (RBB) after 2 days of incubation at 100 ppm dye concentration. In conclusion, it is recommended that fungi isolated from hypersaline environments can be used as potential decolorization agents in the treatment of industrial wastewater containing dye and salt.