Objective: Infertility is shown in approximately 10-15% of the couples. Male infertility is responsible for at least 50% of them. In these infertile couples, male is responsible alone in 30% while male and female are responsible together in 20%. The semen analysis is the first laboratory test which should be done to evaluate the male infertility. Heavy metals and trace elements affect sperm production, motility and morphology. The aim of this study is to determine zinc, copper, lead and cadmium levels in blood and seminal samples of men and to evaluate their relationship with sperm quality parameters. Material and Methods: Forty-two men with abnormal sperm quality parameters and 10 control subjects were included in the study. Zinc, copper, cadmium and lead levels were measured in blood and semen. The results were compared. Results: Lead levels in blood, cadmium levels both in blood and seminal plasma were significantly higher in men with abnormal sperm analyses (p<0.05). There was a positive correlation between seminal plasma zinc level and sperm count, motility and morphology (p<0.001) and between seminal plasma copper level and sperm count and morphology (p<0.05). There was a negative correlation between lead level and sperm count, motility and morphology (p<0.001). Likewise, there was a negative correlation between blood cadmium level and sperm motility and morphology (p<0.05) and between seminal plasma cadmium level and sperm motility (p<0.05). Conclusion: Zinc and copper affect sperm quality positively whereas lead and cadmium shows a negative effect. Exposure to heavy metals is an important etiology in the male infertility problem and should be treated seriously.