Sclerotium rolfsii Sacc. (the sclerotial state of Athelia rolfsii (Cruzi) Tu and Kimbrough), the soil-borne pathogen on several plants all over the world, has been previously reported from Turkey on certain plants. In this study, turfgrass areas in 9 provinces of Turkey were firstly surveyed for S. rolfsii, and samples showing chlorotic, reddish-brown, and frog-eye shaped circular patches were collected. Totally, 32Sclerotium rolfsii isolates were obtained from these areas. One mycelial compatibility group (MCG) was identified among S. rolfsii isolates. Disease severity in pathogenicity tests carried out in the greenhouse ranged from 83.74 to 92.87%. Identification of fungal and bacterial isolates used in the study was performed by DNA sequencing analysis. Five antagonistic bacterial strains, previously found as effective on controlling some fungal pathogens, were tested to determine their antifungal effects against the southern blight by using seed coating method in greenhouse conditions. In consequence of the biological control studies, Bacillus cereus 44bac and Stenotrophomonas rhizophila 88bfp were found more effective than the other strains with the ratio of 91.00 and 90.11%, respectively.