Akademik Veri Yönetim Sistemi
Fabad Journal of Pharmaceutical Sciences, cilt.34, sa.2, ss.83-89, 2009 (Scopus)
Bak protein, which is one of the proteins stimulating apoptosis, leads to cell apoptosis by causing cytochrome c release from mitochondria membranes and followed by caspase activation. The aim of this study, the aim was to investigate the effect of quercetin, a flavonoid that has several biological activities especially the induction of antiproliferative effect and apoptosis in breast and colon cancer cell lines, on Bak protein. MCF-7 and CaCo-2 cell lines were treated with quercetin doses of 25, 50 and 100 μM. After the treatment, levels of Bak protein was investigated by staining with immunohistochemical method at 24th, 48 th and 72 nd hours. It was found that quercetin increased the Bak percentage at 24, 48, and 72 hours, with the most significant increase in the MCF-7 cell line was with 100 μM quercetin concentration at 48 hours and the most significant increase in the CaCo-2 cell line was with 25 μM quercetin concentration at all time points. In conclusion, it was found that quercetin is effective on levels of Bak protein and can also induce apoptosis via this mechanism and furthermore it was observed that this effect may appear in different cells at different concentrations and hours.