Synthesis and characterization of 4-(2-(4-methoxyphenyl)benzo[b] thiophen-3-yl)benzaldehyde for carbohydrate antigen 125 electrochemical detection and molecular docking modeling

DEMİR KIVRAK H., Er O. F., Ozok Ö., Çelik S., KIVRAK A.

MATERIALS CHEMISTRY AND PHYSICS, cilt.281, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 281
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.matchemphys.2022.125951
  • Dergi Adı: MATERIALS CHEMISTRY AND PHYSICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Chemical Abstracts Core, Communication Abstracts, INSPEC, Metadex, Civil Engineering Abstracts
  • Anahtar Kelimeler: Cancer, Carbohydrate antigen 125, Ovarium, Benzothiophene, Molecular docking, Sensor, TUMOR-MARKERS, GRAPHENE OXIDE, OVARIAN-CANCER, NANOPARTICLES, IMMUNOASSAY, ELECTRODE
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

4-(2-(4-methoxyphenyl)benzo[b]thiophen-3-yl)benzaldehyde (MPBB)-based chemical antibody is developed to detect CA125 (MUC-16) level more effectively via electrochemical methods. Novel MPBB is synthesized by using Pd-catalyst coupling reactions and electrophilic cyclization reactions. New benzothiophene structure is dispersed in Nafion solution and CA125 antigen was incubated on this electrode. Optimum conditions are found as 3 mu L MPBB loading, 30 ng/mL CA125 antigen concentration, and 90 min incubation time. Two distinct linear ranges of the electrode prepared at optimum conditions are determined by DPV as 5-50 ng/ml and 100-500 ng/ml. Limit of detection and limit of quantification values have been obtained as 0.03385 ng/mL and 0.10155 ng/mL, respectively. Interference and artificial serum results reveal that this electrode is a promising electrode for CA125 antigen determination for ovarian cancer. Binding energy is investigated by employing a molecular docking modeling study between CA125 antigen and MPBB. Electrochemical results and molecular docking results revealed that the MPBB is a promising alternative to marketed CA125 immunoassays.