Statistical optimization of cultural variables for enzymatic degradation of aflatoxin B-1 by Panus neostrigosus

Soylemez T., YAMAÇ M., YILDIZ Z.

TOXICON, cilt.186, ss.141-150, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 186
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.toxicon.2020.08.003
  • Dergi Adı: TOXICON
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, Environment Index, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.141-150
  • Anahtar Kelimeler: Aflatoxin B-1, Degradation, Panus neostrigosus, Optimization, Plackett-Burman design, Box-Behnken methodology, DETOXIFICATION, PURIFICATION, DECOLORIZATION, MYCOTOXINS, BACTERIUM, LENTINUS, FEED, CORN, FOOD
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

The aim of this study is to determine the best aflatoxin B-1 degradation conditions which was optimized using a combination of the Plackett-Burman and Box-Behnken methods with Panus neostrigosus culture filtrate. Panus neostrigosus was grown in a modified Kirk Broth medium to determine optimal degradation conditions. As a result, aflatoxin B-1 was degraded under varying culture conditions. The Plackett-Burman method was designed after sixteen different experiments with fifteen variables. The three most effective variables (Sucrose, yeast extract, wheat bran) were chosen for the Box-Behnken methodology. The aflatoxin B-1 degradation rate was 49% in just 1 h exposure to culture filtrate which was obtained under optimal growth conditions; (g-ml/L) sucrose 10, yeast extract 3, wheat bran 3, soytone 5, KH(2)PO(4)2, MgSO4 center dot 7H(2)O 0.5, CaCl2 center dot H2O 0.1, ammonium tartrate 2, trace element solution 10; 28 degrees C of incubation temperature, medium pH 5, 7.5% inoculum rate, 125 rpm of agitation speed, and a twelve-day incubation period. The SDS-PAGE studies show that the enzyme responsible for AFB(1) degradation has 38 kDa molecular weight and has no laccase or MnP activity. To the best of our knowledge, this is the first report for AFB(1) degradation by Panus neostrigosus.