Akademik Veri Yönetim Sistemi
JOURNAL OF CLINICAL AND ANALYTICAL MEDICINE, cilt.6, ss.21-25, 2015 (ESCI)
Aim: Prevention of secondary injury developing as a result of spinal cord injury will reduce significantly neurological deficits which may occur after trauma. These are focused in experimental studies and many agents are being tested. In our study, we have invesitigated the effects of kefir, which is a a probiotic, associated with life prolongation, whose antioxidant and lipid peroxidation effects were revealed on despite the scientific studies were limited, on lipid peroxidation and lysosomal proteases which play important roles in spinal cord. Material and Method: In the study, female Sprague Dawley rats weighing 200 to 250 g were used. The study was conducted on five groups with a total of 40 rats including the control, trauma, trauma+treatment, trauma+ treatment+ kefir and trauma+ kefir groups. The high-dose methylprednisolone was used as the therapy. Spinal cord trauma was performed with clip compression method at the level of T10. Kefir was given to rats via orogastric ways, prior to trauma for 7 days at a dose of 2 * 1cc/100g. All rats were sacrificed 48 hours after treatment. The changes in the value of tissue cathepsins B and L, MDA and histopathological changes were examined. Results: It has been found in our study according to the review of biochemical values that; kefir did not reduce significantly cathepsin B values compared to the treatment group (p> 0.05), did reduce significantly MDA value compared to treatment group (p < 0.05). It has been seen that cathepsin L values did not reduce significantly for both groups. It has been observed in histopathological examination that the trauma+ treatment + kefir and trauma+ kefir groups had near-normal histological structure after trauma. Discussion: It can be said in the present study that kefir could partly prevent the secondary injury in acute spinal cord injury with its neuroprotective properties especially with the antioxidant property. We believe that kefir as a new agent can be used in the treatment of spinal trauma, can bring a new perspective to this experimental study field in terms of being a probiotic.