Optical Investigation of DNA Behaviour in Flow using Confocal Detection Incorporated with Fluorescence Correlation Spectroscopy (FCS)
Journal of Fluorescence, vol.35, no.10, pp.9503-9511, 2025 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 35 Issue: 10
- Publication Date: 2025
- Doi Number: 10.1007/s10895-025-04263-5
- Journal Name: Journal of Fluorescence
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Biotechnology Research Abstracts, Chimica, Compendex, MEDLINE
- Page Numbers: pp.9503-9511
- Keywords: DNA behaviour in Flow, Flow-dominated regime, Fluorescence Correlation Spectroscopy (FCS), Microfluidics, Rheological properties of DNA
- Eskisehir Osmangazi University Affiliated: Yes
Abstract
Investigation of the behavior of biopolymers in flow such as Deoxyribonucleic acid (DNA), is a critical challenge in engineering, polymer, and life sciences. In this study, we studied the rheological properties of DNA and flow characteristics in real-time. The velocity measurement was carried out using confocal detection incorporated with Fluorescence Correlation Spectroscopy (FCS). Optical experiments provided an understanding of the diffusion- and flow-dominated regimes for molecules treated in microfluidic channels and lab-on-chip devices in general. We found that the flow-dominated regime starts at a flow rate of 0.3 µl/min and the transitional regime falls into 0.02–0.3 µl/min flow rates. There are a few examples for the detection of DNA and different fragments in flow as such. It is therefore believed to provide valuable insights into the subject of flow dynamics of DNA.