Optical Investigation of DNA Behaviour in Flow using Confocal Detection Incorporated with Fluorescence Correlation Spectroscopy (FCS)
Journal of Fluorescence, cilt.35, sa.10, ss.9503-9511, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 35 Sayı: 10
- Basım Tarihi: 2025
- Doi Numarası: 10.1007/s10895-025-04263-5
- Dergi Adı: Journal of Fluorescence
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Biotechnology Research Abstracts, Chimica, Compendex, MEDLINE
- Sayfa Sayıları: ss.9503-9511
- Anahtar Kelimeler: DNA behaviour in Flow, Flow-dominated regime, Fluorescence Correlation Spectroscopy (FCS), Microfluidics, Rheological properties of DNA
- Eskişehir Osmangazi Üniversitesi Adresli: Evet
Özet
Investigation of the behavior of biopolymers in flow such as Deoxyribonucleic acid (DNA), is a critical challenge in engineering, polymer, and life sciences. In this study, we studied the rheological properties of DNA and flow characteristics in real-time. The velocity measurement was carried out using confocal detection incorporated with Fluorescence Correlation Spectroscopy (FCS). Optical experiments provided an understanding of the diffusion- and flow-dominated regimes for molecules treated in microfluidic channels and lab-on-chip devices in general. We found that the flow-dominated regime starts at a flow rate of 0.3 µl/min and the transitional regime falls into 0.02–0.3 µl/min flow rates. There are a few examples for the detection of DNA and different fragments in flow as such. It is therefore believed to provide valuable insights into the subject of flow dynamics of DNA.