Determination of Calpastatin (CAST) Gene Polimorphism in Some Native Sheep Breeds Reared in Turkey by PCR-RFLP Method

BALCIOĞLU M. S., KARSLI T., Sahin E., Ulutas Z., Aksoy Y.

JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, vol.20, no.4, pp.427-433, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 4
  • Publication Date: 2014
  • Doi Number: 10.15832/tbd.87050
  • Journal Name: JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.427-433
  • Keywords: Calpastatin gene, Polymorphism, PCR-RFLP, Sheep, MEAT QUALITY TRAITS, POLYMORPHISM, GROWTH, ASSOCIATION, RECEPTOR, CARCASS, CATTLE
  • Eskisehir Osmangazi University Affiliated: No

Abstract

In this study, calpastatin (CAST) gene polimorphism was investigated in 7 native sheep breeds reared in Turkey by using PCR-RFLP method. The frequencies of M and N alleles of CAST gene in Kangal (n= 31), Awassi (n= 26), Guney Karaman (n= 23), Akkaraman (n= 21), Morkaraman (n= 34), Karayaka (n= 33), and Karakas (n= 22) sheep breeds were determined as 0.92-0.08, 0.59-0.41, 0.67-0.33, 0.69-0.31, 0.87-0.13, 0.86-0.14, 0.89-0.11 respectively. According to chi-square test, all the other populations were consistent with Hardy-Weinberg equilibrium, whereas Morkaraman, Ivesi and Karayaka populations showed significant (P<0.05) deviation from Hardy-Weinberg equilibrium for the CAST gene.