Effects of vitamin E supplementation on myofibrillar proteins of fat-tailed awassi male lambs


Aksu M. İ. , Aktas N., Kaya M., Macit M.

JOURNAL OF MUSCLE FOODS, vol.16, no.3, pp.177-183, 2005 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 16 Issue: 3
  • Publication Date: 2005
  • Doi Number: 10.1111/j.1745-4573.2005.07804.x
  • Journal Name: JOURNAL OF MUSCLE FOODS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.177-183
  • Eskisehir Osmangazi University Affiliated: No

Abstract

In this study, the effect of Vitamin E supplementation (for 75 days, 45 mg/day Vit E [DL-alpha tocopheryl acetate]) on myofibrillar proteins in the M. Longissimus dorsi of Awassi male lambs was investigated. The lambs were divided into two groups consisting of a control (CG, n = 12) and an experimental (VG, n = 12) treatment starting at the beginning of the fattening period. Both the control group and the Vitamin E group were fed with a concentrate and grass hay close to ad libitum by biweekly adjustment of the amount offered. In addition, the Vitamin E group received the same concentrate plus a supplement of 45 mg of Vitamin,E (DL-a tocopheryl acetate) per lamb per day for 75 days. The effects of Vitamin E on myofibrillar proteins in the M. Longissimus dorsi were determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The SDS-PAGE gels indicated that Vitamin E supplementation to diet had a significant effect on actin, myosin and tropomyosin. The density of these bands increased in samples from the Vitamin E-supplemented group. Also, the pH values of the M. Longissimus dorsi muscles were determined in the present study, and differences between the control and the Vitamin E-supplemented groups were found insignificant.