A Useful Method for the Detection of Ethylenediaminetetraacetic Acid- and Cold Agglutinin-Dependent Pseudothrombocytopenia

Ozcelik F., Arslan E., SERDAR M. A., Yiginer O., Oztosun M., KAYADİBİ H., ...More

AMERICAN JOURNAL OF THE MEDICAL SCIENCES, vol.344, no.5, pp.357-362, 2012 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 344 Issue: 5
  • Publication Date: 2012
  • Doi Number: 10.1097/maj.0b013e318242603d
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.357-362
  • Keywords: Pseudothrombocytopenia, EDTA, Cold agglutinin, Kanamycin, Citrate, PLATELET ANTIBODIES, SUDDEN ONSET, EDTA, THROMBOCYTOPENIA, PSEUDOLEUKOCYTOSIS, PATIENT
  • Eskisehir Osmangazi University Affiliated: No


Introduction: Pseudothrombocytopenia (PTCP), caused by platelet (PLT) aggregation, is usually associated with ethylenediaminetetraacetic acid (EDTA)-dependent antibodies and cold aggluti-nins against PLT antigens. The aim of this study was to identify the PTCP and discover the most practical method to distinguish it from real thrombocytopenia. Methods: This study included 85 patients without hemorrhagic abnormalities and suspected PTCP. Blood samples containing EDTA, citrate and EDTA-kanamycin (KN) were analyzed at room temperature and 37 degrees C. Results: PTCP was detected in 24 of 85 patients. In 23 of 24 patients, EDTA-dependent pseudothrombocytopenia (EDTA-PTCP) was detected; 5 of whom had also the cold agglutinin-dependent PTCP. In only 1 of 24 patients, the cold agglu-tinin-dependent PTCP was found. In this study, no significant difference was observed in leukocyte counts comparing EDTA and citrate blood samples in cases with EDTA-PTCP. Conclusion: In clinical laboratories, a significant portion of the cases with low PLT counts was attributable to EDTA-PTCP and, therefore, did not require treatment. Even if these cases can be detected by bringing the blood samples containing EDTA to 37 degrees C or by adding KN to blood samples containing EDTA, the use of blood samples containing citrate taken for erythrocyte sedimentation rate analysis is a more practical priority method.