Gamma-aminobutyric acid loaded halloysite nanotubes and in vitro-in vivo evaluation for brain delivery

KIRIMLIOĞLU G. Y., Yazan Y., Erol K., ÇENGELLİ ÜNEL Ç.

INTERNATIONAL JOURNAL OF PHARMACEUTICS, vol.495, no.2, pp.816-826, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 495 Issue: 2
  • Publication Date: 2015
  • Doi Number: 10.1016/j.ijpharm.2015.08.087
  • Journal Name: INTERNATIONAL JOURNAL OF PHARMACEUTICS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.816-826
  • Keywords: Gamma-aminobutyric acid, Halloysite, Nanotube, Epilepsy, Brain delivery, Pentylenetetrazole, CLAY NANOTUBES, SUSTAINED-RELEASE, GABA, IBUPROFEN
  • Eskisehir Osmangazi University Affiliated: Yes

Abstract

Gamma-aminobutyric acid (GABA) is a key neurotransmitter where it usually inhibits impulse transmission. GABA release blockage or postsynaptic reaction were determined to provoke epileptic convulsions. The aim of the present study was the development of brain-targeted, nanosized, nontoxic, biocompatible, highly specific formulations. Incorporation of GABA into halloysite nanotubes (HNT) was performed using different methods. Particle size, zeta potential and pH measurements, morphological, thermal, XRD, FTIR analyses and GABA quantification by validated HPLC method were used for the characterization of the systems prepared. Release pattern of GABA from the nanotubes was determined using a dialysis membrane.