SCREENING OF ALCOHOL DEHYDROGENASE ENZYME ACTIVITY OF MICROFUNGI ISOLATED FROM A HYPERSALINE ENVIRONMENT, LAKE TUZ, TURKEY

YENİCE GÜRSU B., Llhan S., ÇALIŞKAN F.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.26, sa.11, ss.6525-6531, 2017 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 11
  • Basım Tarihi: 2017
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.6525-6531
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Extremophile microorganisms can survive in many different environments such as high salt concentrations, high temperatures, high pressures and extreme pHs. They generate unique industrial biocatalysts, run in the extreme conditions. Similarly, Alcohol Dehydrogenases (ADH) from halophilic/halotolerant fungi are exciting biocatalysts with potential industrial applications. In this study, the microfungi isolated from Lake Tuz (Salt Lake or Tuz Golu) in Turkey were investigated in terms of ADH enzyme activity. Primarily, 38 fungal isolates were screened for ADH enzyme activity and then eight isolates with the highest activity were identified by molecular methods with sequencing of internal transcribe spacer regions. These species are the following; Cladosporium macrocarpum, Eurotium niveoglacum, Aspergillus versicolor, A. niger, C. sphaerospermum, C. cladosporioides, Penicillium chrysogenum and E. amstelodami. Specific enzyme activity of these species ranged from 3.21 to 5.93 U/mg.