Biotechnological potential of fungi isolated from Acıgöl/Turkey, the second largest alkaline lake in the world


Ocak E. , İlhan S. , İrdem E., Özbiçen E., Usakbek Kyzy L., Demirel R., ...Daha Fazla

3rd International Eurasian Conference on Biological and Chemical Sciences, Ankara, Türkiye, 19 - 20 Mart 2020, ss.303

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.303

Özet

Haloalkalitolerant fungi have inherent ability to grow and thrive under polyextreme conditions. The bioactive compounds such as enzymes, alkaloids, peptides, antibiotics, exopolysaccharides, and proteins produced and released under stressful conditions have potential biotechnological applications especially in agriculture, food, health care, and medicine. Thus, microfungi isolated from Acıgöl Lake/Turkey, were screened to reveal their antimicrobial and antioxidant properties, some hydrolytic enzymes activity and their availability in extracellular silver nanoparticle production via green synthesis. In this study, 59 isolates were screened for biotechnological potentials. The agar diffusion method was used to determine the antimicrobial activities and the DPPH radical scavenging method was used to determine antioxidant activities. Starchiodine method, 1% tributyrin plate assay, skim milk plate assay, carboxymethyl cellulose plate method were used for determining amylolytic, lipolytic, proteolytic and cellulolytic activity, respectively. As a result, 26% of the isolates showed antibacterial activity while 20% showed antifungal activity. Free radical scavenging activities of the isolates were evaluated against positive control (BHT) and it was calculated in percent. Four isolates were found to have a level (90% and above) that can compete with positive control. Almost all the isolates (82%) showed lipolytic activity. It was determined that especially 12 isolates had a significant level of activity. 22% of the isolates showed amylolytic activity, more prominent in 3 of them. 33% of the isolates showed cellulolytic activity and high activity was observed in 6 isolates. While 68% of the isolates showed proteolytic activity, it was determined that especially 7 isolates had a significant level of activity. It is thought that the isolates are not suitable for extracellular silver nanoparticle synthesis. When all the results are considered, it has been determined that the isolates have the potential to serve in different industrial areas.