3rd International Eurasian Conference on Biological and Chemical Sciences, Ankara, Türkiye, 19 - 20 Mart 2020, ss.303
Haloalkalitolerant fungi have inherent ability to grow and thrive under polyextreme conditions. The bioactive compounds
such as enzymes, alkaloids, peptides, antibiotics, exopolysaccharides, and proteins produced and released under stressful
conditions have potential biotechnological applications especially in agriculture, food, health care, and medicine. Thus,
microfungi isolated from Acıgöl Lake/Turkey, were screened to reveal their antimicrobial and antioxidant properties, some
hydrolytic enzymes activity and their availability in extracellular silver nanoparticle production via green synthesis.
In this study, 59 isolates were screened for biotechnological potentials. The agar diffusion method was used to determine
the antimicrobial activities and the DPPH radical scavenging method was used to determine antioxidant activities. Starchiodine method, 1% tributyrin plate assay, skim milk plate assay, carboxymethyl cellulose plate method were used for
determining amylolytic, lipolytic, proteolytic and cellulolytic activity, respectively.
As a result, 26% of the isolates showed antibacterial activity while 20% showed antifungal activity. Free radical scavenging
activities of the isolates were evaluated against positive control (BHT) and it was calculated in percent. Four isolates were
found to have a level (90% and above) that can compete with positive control. Almost all the isolates (82%) showed
lipolytic activity. It was determined that especially 12 isolates had a significant level of activity. 22% of the isolates showed
amylolytic activity, more prominent in 3 of them. 33% of the isolates showed cellulolytic activity and high activity was
observed in 6 isolates. While 68% of the isolates showed proteolytic activity, it was determined that especially 7 isolates
had a significant level of activity. It is thought that the isolates are not suitable for extracellular silver nanoparticle synthesis.
When all the results are considered, it has been determined that the isolates have the potential to serve in different industrial