Insecticidal effect of entomopathogenic nematodes and the cell-free supernatants from their symbiotic bacteria against different larval instars of Agrotis segetum (Denis & Schiffermuller) (Lepidoptera: Noctuidae)

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Yuksel E., İMREN M., ÖZDEMİR E., BOZBUĞA R., CANHİLAL R.

EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL, cilt.32, sa.1, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1186/s41938-022-00555-1
  • Dergi Adı: EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Arab World Research Source, BIOSIS, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Anahtar Kelimeler: Biological control, Steinernema feltiae, Heterorhabditis bacteriophora, Xenorhabdus sp, Photorhabdus sp, Agrotis segetum, HETERORHABDITIS-BACTERIOPHORA, STEINERNEMA SPP., LIFE-CYCLE, HOST, MOTH
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Background Entomopathogenic nematodes (EPNs) (Steinernematidae and Heterorhabditidae) and their symbiotic bacteria are pathogenic for a wide range of insect pests and have been used successfully as a biological control agent. Although EPNs are well studied against many agricultural insect pests, the efficacy of their symbiotic bacteria still remains unclear for many insect pests of agricultural importance. In the present study, the virulence of native EPN isolates and their cell-free supernatants of symbiotic bacteria were tested against the 3rd and 4th larval instars of Agrotis segetum (Denis & Schiffermuller) (Lepidoptera: Noctuidae) under laboratory conditions (25 +/- 1 degrees C and R.H. 60%). Results The 4th instar larvae were more susceptible to infective juveniles (IJs) and mortalities over (95%) were achieved by all tested EPN isolates at the concentration of 100 IJs/cm(2) after 72 hrs of exposure. The cell-free supernatants were more effective against the 3rd instar larvae and the highest mortalities were recorded as 42 and 60% in the contact and leaf disc bioassays, respectively. Conclusion The results indicated that the cell-free supernatants can be an ideal application for young larval stages of A. segetum. However, further studies are required to test the effectiveness of both EPNs and the cell-free supernatants of their symbiotic bacteria in field conditions.