Objective: Scavenging of free radicals and inhibition of lipoxygenase are important target in the treatment of a variety of inflammatory diseases. Calamintha nepeta (L.) Savi subsp. glandulosa (Req.) P.W. Ball of Lamiaceae was previously reported in the treatment of several diseases in folk medicine. In this study, the essential oil of C. nepeta subsp. glandulosa was investigated for its radical scavenging activity and inhibitory effect on lipoxygenase.