A precise and accurate flow injection analysis (FIA) method for the determination of mitomycin-C (MMC) in pharmaceutical flacons is described. Britton-Robinson (BR) buffer at pH 7.0 was used as a carrier solvent at a flow-rate of 1.0 mL min(-1). MMC signals were detected at 363 nm using a UV-spectrophotometric detector. The intra- and inter-assay precisions were less than 2.0%. The calibration curves were also linear in the concentration range of 2.0 x 10(-6) -1.0 x 10(-5) M for intra- and inter-day studies with high correlation coefficients. The limit of detection (LOD) and limit of quantification (LOQ) values were found to be 1.93 x 10(-8) and 6.45 x 10(-8) M, respectively. The results obtained with the application of the method were compared with those of high performance liquid chromatography (HPLC) and UV-spectrophotometry. It was found that there was insignificant difference between the methods.