Immunological approaches to plant cell wall and biomass characterization: Immunolocalization of glycan epitopes

Avci U., Pattathil S., Hahn M. G.

Methods in Molecular Biology, cilt.908, ss.73-82, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 908
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/978-1-61779-956-3_7
  • Dergi Adı: Methods in Molecular Biology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.73-82
  • Anahtar Kelimeler: Biomass, Cell wall glycans, ELISA, Epitope, Fluorescence, Immunolocalization, Microscopy, Monoclonal antibody
  • Eskişehir Osmangazi Üniversitesi Adresli: Hayır

Özet

Plant cell walls are dynamic structures that show changes in composition and configuration depending on the developmental stage, biotic, and abiotic factors. Therefore, it is necessary to have tools for visualizing the components of the cell wall in situ at any stage. Here, we describe how specific monoclonal antibodies can be used to examine the distribution of plant cell wall glycan epitopes at the whole plant, tissue, cell, and subcellular levels. Understanding the basic cell wall structure is essential for devising efficient strategies to convert cell walls to fermentable sugars for ethanol production. © 2012 Springer Science+Business Media, LLC.