The effect of different hormone concentrations and dark pretreatment on adventitious shoot regeneration in snake melon (Cucumis melo var. flexousus)

Mendi Y. Y., Comlekcioglu N., Ipek M., Kocaman E., Izgu T., Tekdal D., ...Daha Fazla

Romanian Biotechnological Letters, cilt.15, sa.4, ss.5392-5395, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 4
  • Basım Tarihi: 2010
  • Dergi Adı: Romanian Biotechnological Letters
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.5392-5395
  • Eskişehir Osmangazi Üniversitesi Adresli: Hayır

Özet

Explants consisting of the proximal part of cotyledons from 7 day-old seedlings, germinated under dark and light conditions, were cultured on MS media containing different concentrations of BA (0, 0.5, 1.0, 2.0 mg/l) and IAA (0, 0.25, 0.5, 1.0 mg/l). Germination ratio of the seeds under both light and dark conditions was found as 66.8 %, but shoot formation ratio obtained from the seeds germinated under dark condition was higher than the seeds germinated under light condition. The highest frequency of adventitious shoot regeneration (42.8 %) was obtained on MS medium with 1.0 mg/l BA+ 0.25 mg/l IAA from the seeds germinated under dark condition. 1.0 mg/l BA+ 0.5 mg/l IAA (34.8 %), 1.0 mg/l BA(32.8 %), 2.0 mg/l BA+ 0.25 mg/l IAA (12.8 %) and 2.0 mg/l BA+ 0.5 mg/l IAA (7.1 %) resulted in a lower shoot regeneration frequency. In the case of the seeds germinated under light conditions, the maximum frequency of adventitious shoot regeneration from cotyledon explants (2.8 %) was achieved on MS medium with 1.0mg/l BA+ 0.25 mg/l IAA. Hormonal supplements of 2.0 mg/l BA (1.8 %) and 2.0 mg/l BA+0.5 mg/l IAA (1.4 %) resulted in a much lower shoot regeneration frequency. © 2010 University of Bucharest.