Akademik Veri Yönetim Sistemi
2. INTERNATIONAL CANKAYA SCIENTIFIC STUDIES CONGRESS, Ankara, Türkiye, 28 - 29 Eylül 2023, ss.1059-1061
SUMMARY Despite significant scientific and technological advances in medical interventions in intensive care, acute renal failure remains a major cause of morbidity and mortality, which has not decreased for many years. Trials to prevent acute renal failure have generally been unsuccessful; unfortunately, dialysis remains the only effective treatment. Therefore, development of new therapeutic modalities for acute renal failure is an area of intense interest. Renal ischemia initiates a complex and interrelated cascade of events that results in kidney cell damage and cell death. Reperfusion is necessary for the survival of ischemic renal tissue. Reperfusion causes additional damage. Ischaemia/Reperfusion (I/R) causes oxidative stress and tissue damage due to an increase in free oxygen radicals. This condition is clinically important because it is frequently observed in some surgical procedures, such as kidney transplantation and aortic aneurysm, and has a high mortality rate. In many studies, it has been found that active substances, such as Melatonin and Pyrrolidine, reduce oxidative damage in the tissue due to their antioxidant effects. Surgical nephrectomy also increases oxidative stress. In this study, we aimed to investigate the effects of Melatonin and Pyrrolidine, which have antioxidant properties, after renal I/R injury in nephrectomized rats.In our study, 40 Sprague Dawley rats were divided into 5 groups of 8 rats each. Right nephrectomy was performed in all groups. 1st Group: Control, 2nd Group: SF+I/R (SF: Saline), 3rd Group: Melatonin+I/R, 4th Group: Pyrrolidine+I/R, 5th Group: Melatonin+Pyrrolidine+I/R. Melatonin (10 mg/kg/day) and pyrrolidine (100 mg/kg/day) were administered intraperitoneally as a single daily dose for 7 days after a 15-day recovery period. All groups except for the control group underwent ischemia for 45 min and reperfusion for 24 h after ischemia. The blood samples were collected intracardially. Malondialdehyde (MDA), Superoxide dismutase (SOD), Glutathione Peroxide Dismutase (GPx), Catalase (CAT) levels were determined. The values were statistically evaluated by one-way ANOVA and Tukey tests in SPSS 18.0 for Windows package program. Kidney tissues were examined under light microscope in 100-500 μm sections after staining with Haematoxylin-Eosin method. Compared to the I/R group, MDA was decreased in the Melatonin and Pyrrolidine alone groups (p=0.002, p=0.01), and SOD was decreased in the Melatonin+Pyrrolidine group (p=0.02). Pyrrolidine alone and Melatonin+Pyrrolidine increased GPx levels (p=0,02, p=0,005). In histological examinations, it was observed that antioxidants decreased degenerative changes; pyrrolidine administered alone was better than melatonin, and the best protective effect was observed with Melatonin+Pyrrolidine. In conclusion, Melatonin and Pyrrolidine have antioxidant effects on renal I/R injury. Keywords: Ischemia/reperfusion, nephrectomy, rat, antioxidant.