In the present study, N-(benzhydryl/thiazol-2-yl)-2-(piperidin-1-yl)acetamide derivatives were obtained by the reaction of 2-chloro-N-(benzhydryl/thiazole-2-yl)acetamides with piperidine derivatives. The chemical structures of the compounds were elucidated by IR, H-1-NMR, and mass spectral data and elemental analyses. These compounds were investigated for their antimicrobial effects. The microbiological results revealed that the compounds were more effective against C. albicans (ATCC-22019) than bacteria. Among all compounds (1-9), compound 3 bearing benzhydryl and 4-methylpiperidin-1-yl moieties was found to be the most effective compound against C. albicans. Compound 3 exhibited antibacterial activity against P. aeruginosa with a MIC value of 62.5 mu g/mL, whereas streptomycin showed antibacterial activity against P. aeruginosa with a MIC value of 125 mu g/mL. Furthermore, compound 3 exhibited the same level of antibacterial activity against S. aureus when compared with streptomycin. Other derivatives did not show significant activity against the tested bacteria.