Akademik Veri Yönetim Sistemi
40th ANNUAL MEETING OF THE EUROPEAN SOCIETY FOR PAEDIATRIC INFECTIOUS DISEASES (ESPID), ATİNA, Yunanistan, 09 Mayıs 2022, cilt.1, sa.217, ss.217
Central nervous system infections are still one of the most important causes of mortality and morbidity in children. In diagnosis, cerebrospinal fluid direct microscopy, biochemical examination, culture, and moleculer tests such as Multiplex PCR panel are used. In this study, the clinical and epidemiological features of pediatric patients were evaluated with central nervous system infections detected by Multiplex PCR method in cerebrospinal fluid.
Thirty-two patients were included in Eskişehir Osmangazi University Pediatric Department between 2018-2021 years , who were diagnosed central nervous system infection with Meningitis/Encephalitis Panel Multiplex PCR in cerebrospinal fluid.
Thirty-two pathogen was detected in 238 cerebrospinal fluid sample by Meningitis/Encephalitis Multiplex PCR. The mean age of the cases was 55 months and 58% were male. The most common symptoms were fever, decreased feeding, confusion, headache, and vomiting. In laboratory tests; leukocytosis 13 (41%), neutrophilia 12 (38%), lymphopenia 10 (32%), elevated crp levels 20 (65%) were found. Findings in cerebrospinal fluid examination were; pleocytosis 30%, neutrophilia 16%, low glucose 16% and high protein 25%. Detected pathogens were Human Herpes Virus-6 in 11 cases, Enterovirus 8, Streptococus pneumonia 5, Varicella Zoster 2, Cytomegalovirus 2, Haemophilus influenza 1, Streptococcus agalactiae 1, Listeria monocytogenes 1, Staphylococcus epidermidis 1. There was growth in cerebrospinal culture in five of 32 cases.The most commonly used antimicrobial therapy were ceftriaxone/cefotaxime, vancomycin, ampicillin and acyclovir. Empirical antibiotic treatment was stopped in eight cases due to the detection of virus by Multiplex PCR.
The most common pathogens in central nervous system infections in children are viruses and bacteria. Multiplex PCR is one of the most specific methods for detecting pathogens especially viral agents. With the use of Multiplex PCR method, the unnecessary use of anthobiotics has been reduced.