Oat breeding activities at the Transitional Zone Agricultural Research Institute (TZARI) have been continuing since 1929. Within the scope of this study, it is aimed to select those that are high in yield and quality from local oat lines that are compatible with regional conditions and to register them as new oat cultivars. Field trials were conducted in rainfed conditions in Eskişehir TZARI Central and Hamidiye Campuses in the 2017-2018 crop season. In the study, ninehteen local lines (Avena sativa L.) collected from different parts of the country and Checota, Kahraman, Kırklar, Yeniçeri and Sebat varieties as a standard were used. In this research, besides various agro-morphological traits such as grain yield (GY), thousand kernel weight (TKW), plant height (PH), lodging score (LS), heading days (HD), grain protein content (PC), beta-glucan (BG), acid detergent fiber (ADF) and neutral detergent fiber (NDF) values were examined. According to the results of this study, differences between genotypes in parameters other than ADF and NDF values were found statistically significant. The means of grain yield was 2,93 t ha-1, and the GY of genotypes was ranged from 2,31 to 3,58 t ha-1 in the trial. TKW was ranged from 25,5 to 37,1g; PH was 83,3 to 130,2 cm; HD was 126,0 to 141,0 days; BG was 3,29 to 5,16%; PC was 13,4 to 15,9%; ADF was 16,4 to 19,4%; NDF was 30,8- 38,8% in the genotypes. Accordingly, G13, G16, G19, G21,G22 and G23 local oat lines have been found to be promising in terms of targeted agro-morphological and quality characteristics and transferred to advanced level in the oat breeding program.