Protective effects of silymarin against acetaminophen-induced hepatotoxicity and nephrotoxicity in mice
TOXICOLOGY AND INDUSTRIAL HEALTH, vol.32, no.4, pp.589-600, 2016 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 32 Issue: 4
- Publication Date: 2016
- Doi Number: 10.1177/0748233713502841
- Journal Name: TOXICOLOGY AND INDUSTRIAL HEALTH
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.589-600
- Keywords: Acetaminophen, silymarin, hepatotoxicity, kidney toxicity, nitric oxide, INDUCED LIVER-DAMAGE, INDUCED RENAL INJURY, NF-KAPPA-B, DNA FRAGMENTATION, REACTIVE OXYGEN, CELL-DEATH, INDUCED CARDIOTOXICITY, LIPID-PEROXIDATION, OXIDATIVE DAMAGE, MILK THISTLE
- Eskisehir Osmangazi University Affiliated: Yes
Abstract
This study was designed to estimate protective effects of silymarin on acetaminophen (N-acetyl-p-aminophenol, paracetamol; APAP)-induced hepatotoxicity and nephrotoxicity in mice. Treatment of mice with overdose of APAP resulted in the elevation of aspartate aminotransferase (AST), alanine transaminase (ALT), blood urea nitrogen (BUN), and serum creatinine (SCr) levels in serum, liver, and kidney nitric oxide (NO) levels and significant histological changes including decreased body weight, swelling of hepatocytes, cell infiltration, dilatation and congestion, necrosis and apoptosis in liver, and dilatation of Bowman's capsular space and glomerular capillaries, pale-stained tubules epithelium, cell infiltration, and apoptosis in kidney. Posttreatment with silymarin 1 h after APAP injectionfor 7 days, however, significantly normalized the body weight, histological damage, serum ALT, AST, BUN, SCr, and tissue NO levels. Our observation suggested that silymarin ameliorated the toxic effects of APAP-induced hepatotoxicity and nephrotoxicity in mice. The protective role of silymarin against APAP-induced damages might result from its antioxidative and anti-inflammatory effects.