Akademik Veri Yönetim Sistemi
5th Internatıonal Balkan Agriculture Congress, Edirne, Türkiye, 20 - 23 Eylül 2023, ss.285-291
The genus Rhizoctonia consists of species that have one, two
(binucleate) and multinucleate hyphae that are pathogenically, morphologically
and genetically different from each other. These species are named and divided
into subgroups depending on the anastomosis reactions between their hyphae and
they show different pathogenic characteristics according to their hosts.
Binucleate Rhizoctonia spp. generally live in soil and plant debris as
saprophytes, but some form parasitic relationships with plants. In this study, surveys were conduct in parks in Kocaeli provinces in
2022. As a result of isolations from 52 turfgrass plants, two binucleate Rhizoctonia AG P isolates were
identified in accordance with the rDNA-ITS sequences analysis. The rDNA internal transcribed spacer
(ITS) regions of the isolates were amplified by polymerase chain reaction (PCR)
using universal fungal rDNA primer pair ITS1/ITS4. The
ITS1-5.8S-ITS2 region of isolates were sequenced and performed BLAST analyzes. Pathogenicity tests were
performed two times in pots using turfgrass seeds in greenhouse conditions. As a result of the pathogenicity tests, it was
determined that both isolates cause damping-off on turfgrass.