Development of potentiometric biosensor for diagnosis of prostate cancer

Altunkok N., Unluer O. B., BİRLİK ÖZKÜTÜK E., ERSÖZ A.

MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS, cilt.263, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 263
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.mseb.2020.114789
  • Dergi Adı: MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Chemical Abstracts Core, Communication Abstracts, INSPEC, Metadex, Civil Engineering Abstracts
  • Anahtar Kelimeler: Sarcosine, Biosensor, GFOX, Prostate cancer, ANADOLUCA method, SARCOSINE OXIDASE, SENSOR, URINE, NANOPARTICLES, ELECTRODE, SERUM
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Prostate cancer is an important public health problem in the world with its burden of disease, increase in fatality and incidence rates. Recently, naturally occurring amino acid sarcosine has been investigated as a recognized marker of prostate cancer. The aim of the study for the early detection of prostate cancer is to develop a method of high sensitivity, low cost and low concentration in the determination of sarcosine. High sensitive nano antisarcosine-antibody and nano anti-sarcosine antibody-GFOX polymer have been prepared for the potentiometric detection of sarcosine and its reusability as biosensors have investigated. Nano anti-sarcosine antibody and nano anti-sarcosine antibody-GFOX potentiometric biosensor have shown excellent detection performance. Nano antisarcosine antibody potentiometric biosensor and nano anti-sarcosine antibody-GFOX potentiometric biosensor have low detection limit (4.75 x 10(-9) mM and 3.34 x 10(-9)mM, respectively), high selectivity, short response time, wide linear range, and satisfactory long-term stability (3-4 months) for sarcosine detection.