A comparison between two different automated total 25-hydroxyvitamin D immunoassay methods using liquid chromatography-tandem mass spectrometry

KOÇAK F. E., Ozturk B., Isiklar Ö. Ö., Genc O., Unlu A., Altuntas I.

BIOCHEMIA MEDICA, vol.25, no.3, pp.430-438, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 3
  • Publication Date: 2015
  • Doi Number: 10.11613/bm.2015.044
  • Journal Name: BIOCHEMIA MEDICA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.430-438
  • Keywords: 25-Hydroxyvitamin D, chromatography, immunoassay, methods, tandem mass spectrometry, VITAMIN-D ASSAYS, PERFORMANCE, ACCURACY
  • Eskisehir Osmangazi University Affiliated: No


Introduction: Total 25-hydroxyvitamin D [25(OH)D] is the most reliable indicator of vitamin D status. In this study, we compared two automated immunoassay methods, the Abbott Architect 25-OH Vitamin D assay and the Roche Cobas Vitamin D total assay, with the liquid chromatography-tandem mass spectrometry (LC-MS/MS).