Investigation of the MMP2 and MMP9 gene polymorphisms in malignant mesothelioma and other pleural diseases

Tuncel T., Metintaş M., Yağcı E., Ak G., Metintas S., Güneş H. V.

Biomedical Research-India, vol.28, no.20, pp.8670-8674, 2017 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 28 Issue: 20
  • Publication Date: 2017
  • Journal Name: Biomedical Research-India
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Academic Search Premier, Chemical Abstracts Core
  • Page Numbers: pp.8670-8674
  • Eskisehir Osmangazi University Affiliated: Yes

Abstract

Various impacts of Matrix Metalloproteinases (MMPs) were reported in many cancer types.

Particularly, MMPs are involved in extracellular matrix destruction which is required cancer cells to

make an invasion. Gene polymorphisms which are thought to affect expressions levels of MMPs are

widely studied to determine their influence on cancer. In this study, we aimed to investigate the

association of gene polymorphism frequencies of MMP2 C1306T and MMP9 C1562T in Malignant

Mesothelioma (MM), other metastatic cancers to pleura and benign pleural diseases. DNA extracted

from pleural tissues of 195 subjects (MM, other metastatic cancers to pleura and benign pleural

diseases). MMP2 C1306T and MMP9 C1562T polymorphisms genotyped using restriction fragment

length polymorphism (RFLP) method. We found no significant difference in genotype frequencies of

MMP2 C1306T, MMP9 C1562T between subjects except for MMP2 C1306T CT genotypes were high in

MM when compared to benign pleural diseases group. We recommend extensive mutation and

polymorphism scan including whole exomes of these genes.