Akademik Veri Yönetim Sistemi
APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH, cilt.18, sa.1, ss.289-302, 2020 (SCI-Expanded)
The aim of this study was to investigate changes of phenolic accumulation and antioxidant potential of goldenberry fruits collected from diploid plants, under colchicine treatments of different doses and exposure time, including colchicine induced synthetic tetraploid plants. Three colchicine application methods were utilized; i) immersion of seeds in colchicine solution at concentrations of 0.0, 0.6, 0.9% for 24 h and 36 h; ii) keeping germinated seeds (with 2-3 mm long roots) in a colchicine solution with concentrations of 0.6% and 0.9% for 24 h iii) incubation of seeds on semi solid nutrient medium containing 0 6% and 0.9% colchicine for 14 and 21 days. Total antioxidant activity of fruits varied according to the dose and exposure time of colchicine applications. The most important change was observed in the fruits of plants that turned tetraploid as a result of colchicine applications. Tetraploid fruits generally produced higher amounts of secondary compounds compared to diploid progenitors and their antioxidant capacity was also higher. However, the results show that polyploidization is important but cannot be generalized as a strategy to increase secondary metabolite production. It was determined that the relationship between total anthocyanin and total phenolic content with antioxidant capacity of fruits was significant and parallel. We suggest the use of the lowest colchicine concentration tested. 0.6% colchicine to enhance metabolite production cannot be generalized.