Effects of silver nitrate and donor plant growing conditions on production of pepper (Capsicum annuum L.) haploid embryos via anther culture

Buyukalaca S., ÇÖMLEKÇİOĞLU N. , Abak K., Ekbic E., Kilic N.

EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE, vol.69, no.5, pp.206-209, 2004 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 69 Issue: 5
  • Publication Date: 2004
  • Page Numbers: pp.206-209
  • Keywords: pepper, androgenesis, pollen embryogenesis, haploid embryo, SHOOT REGENERATION, ETHYLENE ACTION, INHIBITOR, EMBRYOGENESIS, IMPROVEMENT, AGNO3


The responses of pepper anthers to different concentrations of silver nitrate and in dependence of the donor plant growing conditions were studied for haploid embryo production via anther culture. U-247 and U-238 genotypes, which were selected from a local paprika pepper population, were used as plant materials. Flower buds were collected from the plants grown either in greenhouse or in open field conditions. Four different silver nitrate concentrations (5, 10, 15 and 20 mg 1(-1)) were tested. Haploid embryos were obtained from all the concentrations tested, but with different production rates. The highest embryo formation (45.7 embryos per 100 anthers) was obtained from the genotype U-247 in the medium containing 15 mg 1(-1) silver nitrate. The anthers taken from the plants grown in the greenhouse produced more embryos than that of the open field conditions.