Possible Curative Effects of Boric Acid and Bacillus clausii Treatments on TNBS-Induced Ulcerative Colitis in Rats

Özkoç M., Can B., Şentürk H., Burukoğlu Dönmez D., Kanbak G.

BIOLOGICAL TRACE ELEMENT RESEARCH, vol.201, no.3, pp.1237-1251, 2023 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 201 Issue: 3
  • Publication Date: 2023
  • Doi Number: 10.1007/s12011-022-03215-5
  • Journal Name: BIOLOGICAL TRACE ELEMENT RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Page Numbers: pp.1237-1251
  • Keywords: Inflammatory bowel diseases, Ulcerative colitis, Boric acid, Bacillus clausii, Probiotics, IL-35, INFLAMMATORY-BOWEL-DISEASE, INTESTINAL ALKALINE-PHOSPHATASE, NITRIC-OXIDE SYNTHASES, GLUTATHIONE-PEROXIDASE, LIPID-PEROXIDATION, OXIDATIVE STRESS, DAMAGE, MICE, MYELOPEROXIDASE, RESVERATROL
  • Eskisehir Osmangazi University Affiliated: Yes

Abstract

Crohn's disease (CD) and ulcerative colitis (UC) are two chronic relapsing inflammatory bowel diseases (IBD). Although there are several treatment options available to improve the symptoms of IBD patients, there is no effective treatment that provides a definitive solution. In the present study, we aim to investigate the antioxidative/anti-inflammatory effects of oral administration of boric acid and Bacillus clausii in a rat trinitrobenzenesulfonic acid (TNBS)-induced colitis model. The effects of boric acid and B. clausii were examined in serum and colon tissues with the help of some biochemical and histological analyses. Elevated inflammation and oxidative damage were found in the blood and colon tissue samples in the TNBS-induced group according to the complete blood count (CBC), tumor necrosis factor (TNF) alpha, interleukin-35 (IL-35), malondialdehyde (MDA), glutathione peroxidase (GPx), myeloperoxidase (MPO), nitric oxide (NO), and histological findings. Particularly, the highest IL-35 level (70.09 +/- 12.62 ng/mL) in the combined treatment group, highest catalase activity (5322 +/- 668.1 U/mg protein) in the TNBS-induced group, and lower relative expression of inducible nitric oxide synthase in the TNBS-induced group than the control group were striking findings. According to our results, it can be concluded that boric acid showed more curative effects, even if B. clausii probiotics was partially ameliorative.