Labeling of mixed leukocytes with Tc-99m-HMPAO causes severe chromosomal aberrations in lymphocytes

Ak I., Vardereli E., Durak B., Gulbas Z., Basaran N., Stokkel M., ...Daha Fazla

JOURNAL OF NUCLEAR MEDICINE, cilt.43, sa.2, ss.203-206, 2002 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 2
  • Basım Tarihi: 2002
  • Dergi Adı: JOURNAL OF NUCLEAR MEDICINE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.203-206
  • Anahtar Kelimeler: Tc-99m-HMPAO, leukocyte labeling, radiation damage, chromosomal aberrations, lymphocytes, IN-111
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Tc-99m-d,1-hexamethylpropyleneamine oxime (HMPAO) is widely used as a labeling agent for leukocytes in the diagnosis of inflammatory or infectious foci. Cytotoxicity studies have indicated that intracellular labeling of leukocytes with In-111 compounds may have severe detrimental effects on the cells. Methods: In this study, the radiotoxic effects on mixed lymphocytes after labeling with Tc-99m-HMPAO was investigated using the cytokinesis-blocked micronucleus assay and chromosomal aberration assay. Results: Whereas negligible numbers of chromosome abnormalities were noted in unlabeled lymphocytes, the labeled lymphocytes showed multiple aberrations of various types, including dicentric, tricentric, and fivecentric chromosomes; centric rings; chromosome and chromatid type breaks; and acentric fragments. Conclusion: Heavily aberrant lymphocytes are seen in Tc-99m-HMPAO-labeled mixed leukocytes after routine clinical procedures. It is unlikely, however, that this would cause detrimental effects, such as lymphoid malignancy, as these cells would normally be eliminated through apoptosis or phagocytosis.