Mast cell stabilizator and antioxidant effects of epidermal growth factor (EGF) on gastric mucosal injury induced by ethanol in rats

Erkasap S., Erkasap N., Aral E., Koken T., Kahraman A., Aydin Y., ...Daha Fazla

CHINESE JOURNAL OF PHYSIOLOGY, cilt.48, sa.1, ss.1-6, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 48 Sayı: 1
  • Basım Tarihi: 2005
  • Dergi Adı: CHINESE JOURNAL OF PHYSIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1-6
  • Anahtar Kelimeler: epidermal growth factor (EGF), ethanol induced gastric mucosal injury, mast cell, oxidative stress, BLOOD-FLOW, HISTAMINE, STRESS, ULCERATION, DAMAGE, PROSTAGLANDIN, PROTECTION, MECHANISM, STOMACH, LESIONS
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

The role of epidermal growth factor (EGF), a polypeptide containing 53 amino acids, on protection and repair of ethanol-induced gastric mucosal injury was investigated in rats. In addition, the effects of EGF on the gastric damage were evaluated histopathologically. We used 48 Spraque-Dawley rats which were divided three groups as control rats, ethanol treated rats and ethanol+EGF treated rats. The ethanol group,vas given a gastric gavage containing 1 ml of 80% ethanol (v/v) prepared in distilled water. EGF (100 mu g/kg) was given by intragastric gavage 30 min before the administration of ethanol. We studied histopathological evaluation and the histochemical heterogeneity of mast cells and its degree of degranulation. Besides, gastric tissue malondialdehyde (MDA), protein sulfhydryl groups (SH), and protein carbonyl levels were measured. EGF treatment stabilized mast cells degranulation and had lower polymorphonuclear leukocytes (PMNL) infiltration, ulcer index, histamine, and NIDA; protein carbonyl levels were also lower, compared to the non-treated animals. EGF exerts a protective effect on gastric mucosa to ethanol-induced gastric injury probably through antioxidant and mast cell stabilizing mechanism.