The quantity, quality, the seasonal and écologie variation of microfungi in the soil samples collected with the Brown's technique from the five wheat fields of Kirka Vicinity, Eskis,ehir between August 1995 and May 1996 were studied. The dilution plate technique was used for isolation of microfungi. The number of microfungi ranged from 20.000 to 275.000 CFU. A total of 11 genera (Alternaria, Aspergillus, Cladosporium, Gliocladium, Microdochium, Papularia, Penicillium, Rhizopus, Talaromyces, Trichoderma, Verticillium) and 28 species (Alternaria alternata, A. radicina, Aspergillus flavipes, A. ficuum, A. fumigatus, A. niveus, A. nutans, A. parvulus, A. terricola, A. wentii, Cladosporium herbarum, Gliocladium roseum, Microdochium nivale, Pénicillium adametzii, P. allahabadense, P. citrinum, P. commune, P. crateriforme, P. de.cumbens, P. estinogenum, P. lanosum, P. olsonii, P. paneum, P. piceum, P. restrictum, P. rugulosum, P. velutinum, Talaromyces wortmannii) were determined. G. roseum and A. wentii were found as the most common species and both were detected in 5 separate stations. The probability of being new for Turkish soil mycobiota is considerably high for A. nutans, P. allahabadense, P. crateriforme and P. paneum species.