Microbial Transformations of Isophorone by Alternaria alternata and Neurospora crassa


KIRAN İ., ÖZŞEN BATUR Ö., Celik T., İLHAN S., YENİCE GÜRSU B., DEMİRCİ F.

NATURAL PRODUCT COMMUNICATIONS, vol.8, no.1, pp.59-61, 2013 (Peer-Reviewed Journal) identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 1
  • Publication Date: 2013
  • Journal Name: NATURAL PRODUCT COMMUNICATIONS
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.59-61
  • Keywords: Alternaria alternata, Antimicrobial evaluation, Biotransformation, Isophorone, MIC, Neurospora crassa, ANTIMICROBIAL ACTIVITY, ASPERGILLUS-NIGER, BIOTRANSFORMATION, DIISOPHORONE, MONOTERPENES

Abstract

Isophorone (3,5,5-trimethyl-2-cyclohexen-1-one), a monoterpene, and the structurally related 1,8-cineole and camphor, have demonstrated a protective effect against cancer, biological activity against a variety of microorganisms, and anti-oxidant properties. The derivatization of isophorone is, therefore, an important field of xenobiochemistry, pharmacology and toxicology. The aim of this study was to obtain derivatives of isophorone through microbial biotransformation and evaluate the biotransformation metabolites as potential antimicrobial agents. Incubation of isophorone with the fungi Alternaria alternata and Neurospora crassa afforded 4 alpha-hydroxy- and 7-hydroxy-isophorone as transformation metabolites. The antimicrobial activities of isophorone and the metabolites were evaluated in vitro both by using agar dilution and microdilution methods. However, no significant antibacterial activity was observed when compared with those of standard substances.