Akademik Veri Yönetim Sistemi
SEPARATION AND PURIFICATION TECHNOLOGY, cilt.357, 2025 (SCI-Expanded)
Rapid and lower cost sensing plays a crucial role in biotechnology and medical diagnostics. |In this regard, biosensors can be utilized in various applications, including the early diagnosis of diseases, identification of genetic disorders, environmental monitoring, and personalized medicine. These sensors offer significant advantages such as rapid diagnosis and high sensitivity. The interior of the cells such as Deoxyribonucleic Acid (DNA), enzymes, etc. can be examined using biosensors which are integrated into microfluidic chips appeared to be promising. However, a major challenge in these systems is the removal of the cell wall from the solution within the sensor environment. This study aims to remove the lysed Escherichia coli (E. coli) bacterial cell wall from the DNA solution in microfluidic chips using nanofibers. Polyvinylidene fluoride (PVDF) and titanium dioxide (TiO2)/PVDF composite nanofibers were produced for this purpose. The goal of incorporating TiO2 nanoparticles was to prepare the mixed matrix membrane with high filtration efficiency while to get the ability of bacteria DNA passage which can be monitored by the biosensors. An improvement in thermal resistance and confirmation of TiO2 nanoparticle incorporation into PVDF matrix were shown by thermal gravimetric analysis (TGA) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. Wettability studies on the prepared composite nanofiber membrane showed a smaller water contact angle, indicating improved hydrophilicity and enhanced filtration performance. Escherichia coli DH5 alpha as a model Gram-negative bacterium, was used to assess filtration performance using a one channel microfluidic chip with a consistent flow rate of 100 mu L/min. The filtration efficiency was quantified using a McFarland densitometer, and DNA passage ability was assessed using a nanodrop spectrophotometer, showing the filtration efficiency while preserving the capacity for bacterial DNA transmission. These membranes have potential for integration into biosensor-equipped microfluidic devices is emphasized by their comprehensive characterization and modification efforts, which can provide real-time monitoring of bacterial contamination and DNA analysis.