Disk-shaped specimens were prepared from additively (NX and DT), subtractively (MZ), and conventionally manufactured denture base resins (CV). Surface roughness and color coordinates were measured after polishing, simulated brushing, and coffee thermocycling, while surface roughness was also measured before polishing. Polishing reduced the surface roughness of all materials. Brushing and coffee thermocycling increased the surface roughness of only DT. CV had the highest susceptibility to consecutive brushing and coffee thermocycling as it had the highest surface roughness, which was above the clinically acceptable threshold. All materials had similar stainability; only MZ had perceptible color change after brushing. Even though stainability of tested denture base resins was similar, additively or subtractively manufactured computer-aided design and computer-aided manufacturing (CAD-CAM) resins had smoother surfaces after brushing and coffee thermocycling, regardless of the material. Therefore, complete dentures made out of these CAD-CAM resins may have favorable surface properties in the long term.