Association of angiotensin converting enzyme (ACE) gene I/D polymorphism and polycystic ovary syndrome (PCOS)

Bayram B., Kilicci C., ÖNLÜ H., Ozkurt M., ERKASAP N., YILDIRIM E., ...More

GENE, vol.489, no.2, pp.86-88, 2011 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 489 Issue: 2
  • Publication Date: 2011
  • Doi Number: 10.1016/j.gene.2011.08.012
  • Journal Name: GENE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.86-88
  • Keywords: ACE gene I/D polymorphism, polycystic ovary syndrome, ACTIVATOR INHIBITOR-1 4G/5G, WOMEN, PREVALENCE, REDUCTASE, RISK
  • Eskisehir Osmangazi University Affiliated: Yes


This study was conducted in Turkish patients with polycystic ovary syndrome to determine the frequency of I/D polymorphism genotypes of angiotensin converting enzyme gene, and to examine the role of this polymorphism in polycystic ovary syndrome development. Genomic DNA obtained from 200 persons (100 patients with polycystic ovary syndrome and 100 healthy controls) was used in the study. DNA was multiplied by polymerase chain reaction using I and D allele-specific primers. Polymerase chain reaction products were assessed with a charge coupled device (CCD) camera by being exposed to 2% agarose gel electrophoresis. There was statistically significant difference between the groups with respect to genotype distribution (p < 0.001). The D allele frequency was indicated as 68% and I allele was as 32% in the patients, whereas it was 51.5-48.5% respectively in the control group. As a result of our study we may assert that angiotensin converting enzyme gene I/D polymorphism DD genotype should be considered as a genetic marker in polycystic ovary syndrome development in this Turkish study population. (C) 2011 Elsevier B.V. All rights reserved.